ADVANCED DIAGNOSTICS OF MOUSE AND RAT

  • ln vivo body imaging – microCT, luminescence, fluorescence, ultrasound including Echo and 4D, endoscopy
  • High resolution ex vivo imaging (embryos, mineralization of bones, assessment of villi in intestine etc.)
  • In vivo MRI body composition analysis – quantification of body fat-muscle-liquid tissue applicable for metabolism tests and disease severity monitoring
  • Ultrasound comprehensive imaging – 2D&3D, including high resolution probe for mouse embryos. Heart functions measurements with or without challenge (blood pressure, EKV, Power Doppler, Treadmill) – detailed analysis, vascularization of tumors
  • Lung functions measurement, histological analysis and flow cytometry (FCM) of bronchoalveolar lavage
  • Other in vivo comprehensive measurements and special applications: Eye defects – eye morphology and function, intra-ocular or other ultrasound driven injections; ERG; Auditory brain response test; Behavioral tests – open field and various mazes, gait analysis, grip strength, rotarod, intellicages etc.; Calorimetry measurements including circadian rhythms, glucose metabolism tests
  • Blood analysis – flow cytometry (standardized and customized panels), clinical biochemistry & other blood plasma markers (e.g. cytokine multiplex, hormones), hematology including coagulometry
  • Flow cytometry assessment of body liquids, tissues and tumor microenvironment (including advanced immunological analysis, T cells in vitro activation)
  • Detailed histopathology – standard organ macroscopical and microscopical pathology, including special stains, immunohistochemistry, model specific markers, slide scans for later evaluation, customized design
  • MALDI imaging of cells or tissues
  • Mass spectrometry, HPLC and other analytical methods for drug or metabolites assessment – established panels: Steroid hormones, bile acids, amino acids, bio-active amines, lipidomics, drug metabolites, other metabolites (list on demand – approx. 200 molecules)

GLP certified services GLP Standardized Modalities & Key Services

Biochemistry Haematology

Biochemistry & Haematology

GLP certified lab 

GLP certified services:

  • Terminal mice blood sampling under isofluran anesthesy
  • Mice euthanasia
  • Complete blood count and differential
  • Clinical biochemistry measurement on automated analyzer
  • Statistical evaluation of data

Other available tests of the unit are also provided according to highly standardized protocols in GLP-like mode.

Bioimaging Embryology

Bioimaging & Embryology

Bone mineralization deffects, bone healing, dental development, drugs theratogenicity 
Cardiovascular

Cardiovascular

Heart function, blood vessel structure, blood presure, organ vascularization 
Vision

Vision

Retinal structure and function, rethinopathy models 
Hearing & Electrophysiology

Hearing & Electrophysiology

Deafness models, hearing loss assesment 
Histopathology

Histopathology

GLP certified lab 

GLP certified services:

  • Complete mice necropsy including gross pathology assessment and organ collection for further processing
  • Complete tissue processing from appropriate fixation to staining; stainings currently available under GLP:
    • Hematoxylin & Eosin
    • Masson Trichrome
    • PAS staining
    • Oil red staining
    • Sirius red
    • NASDCL staining
    • Perls’ staining of iron ions
  • Evaluation of tissue pathology
  • Slide scanning
  • Statistical evaluation of data

GLP like mode Other Standardized Modalities & Key Services

Other available tests of the unit are also provided according to highly standardized protocols in GLP-like mode.

Immunology

Immunology

Standardized analysis of immune response, tumor microenvironment analysis 
Lung Function

Lung Function

Asthma, fibrosis, emphysema models 
Metabolism

Metabolism

Diabetes, colitis & CAC & CRC, obesity, liver fibrosis (diet or BDL induced) 
Metabolomics

Metabolomics

Drug&metabolites tracking, untargeted screening – off side effects 
Neurobiology and Behaviour

Neurobiology & Behaviour

Autism, side effects targeting motion, coordination 

PDX & Cancer Models

CDX, PDX, mutagen administration, genetic models 
Biostatistics

Biostatistics / Bioinformatics

Barinka cre elpo 400ng_00004

New Genetically Modified Mouse/Rat Disease Model Generation