PDX & Cancer Models

The PDX (patient derived tumor xenograft)/Cancer Models Unit is dedicated to creating novel orthotopic mouse/rat PDX models, which are used in cancer, immunology, and pharmacology research. PDX are models of cancer where the tissue or cells from a patient’s tumor are implanted into a highly immunodeficient or humanized mouse. We use the well-suited NSG and NSG-SGM3 mouse strain to fulfil this purpose. More info

The samples for implantation or xenografts are placed orthotopically and then the tumor development followed. Mouse tumor samples can then be viably stored in our cryobank in parallel to evaluations in the CCP’s histopathology, haematology, biochemistry, immunology, and, bioimaging units as well as the samples can be analyzed using uCT, MALDI imaging and/or metabolomics approaches. PDX mouse models represent therefore a promising research platform for personalized medicine including pharmacological and metabolic studies. The main benefit is a possibility of in-house multimodal analysis of the models.

Standard services Cancer Cell Line Models, Patient Derived Xenograft and Leukemic Models

(Service provided in cooperation with Bioimaging, Biochemistry and Immunology units)

Irradiation Service – Xrad 320

The X-RAD 320 is a self-contained X-ray system for delivering a precise radiation dosage to specimens (cells, tissue, mice, rats, etc.) in a biological or small animal research laboratory. The shielded cabinet includes an adjustable specimen shelf, sample view window and beam hardening filter holder. The TouchRAD control panel is a multi-user, password protected touch screen interface with a transportable database that can track individual system usage.

Cell Culturing

We provide cell culturing for in vivo studies including leukemic cells. Upon the request, the cells might be labelled to facilitate monitoring of tumour development and metastasis throughout whole study. See Bioimaging unit.

We have direct experience with these cell lines: 4T1, HCT 116, LnCap, MCF7, EL4, OVSAHO, KURAMOCHI, EFO27, PEO4, HME1 leukemic cell lines MOLM 13, OCI –M2, SKM, NB4.

4T1 cell line

Orthotopic CDX/PDX Engraftment, Cancer Model Development and Optimization

  • Subcutaneous, Intraductal, Mammary fat pad, Caecal, Subcapsular, Ovary fat pad …
  • Intrafemoral engraftment of leukemic cells
  • As the partners of the EuroPDX consortium, we are able to expand patient derived grafts for your research, we use NSG and NSG-SGM3 mice.
  • On request, we will send you a list of available PDX samples.
Intracaecal application of cells

In vivo Tumour Growth Evaluation

  • Direct measurement – palpation and calliper measurement
  • In vivo imaging – please see overview of used markers in Bioimaging unit
  • Ultrasound – for more ultrasound application see Cardiovascular unit
  • Endomicroscopy in preparation
Orthotopic luciferase reporter-labeled tumor cells
Intrammamary tumor with Luciferase reporter-labeled cells
Ovarium (red) and tumor of ovary fat pad (blue) close to kidney

Animal Health Monitoring

Our day-to-day monitoring the animals allow us to collect important side information to describe and predict of progression of disease and therefore decrease failure rate of clinical trials, improve disease detection studies and customize disease prevention strategies. We commit to a high level of biosafety and sterility to allow us to proceed with long-term studies lasting up to several months without compromising welfare.

Pulse oximeter

In vivo and Aseptic Sampling

Save sampling without additional contamination – blood, urea, cerebrospinal fluid, tissue samples.
For standard necropsy and histology evaluation, please see Histopathology unit.

Viable Cryopreservation of Cell Tumour/Tissue Samples

Statistical Analysis

Study results might be finalised by our bioinformaticians.

Do you have questions? Ask us

Standard services Preclinical Efficacy Studies on PDX (Patient Derived Xenograft) /CDX (Cell Line Derived Xenograft) Models

Service provided in cooperation with Biochemistry and Haematology; Immunology, and Bioimaging units.

Our goal is to reduce the time, cost and failure rate of pharmaceutical clinical trials.

Model Development

See above Cancer cell line models, patient derived xenograft and leukemic models)

Drug Administration

per os, i.m., s.c., i.v., i.f. , into tumour

Daily Clinical Healthy Control Measurements Including Monitoring of Side Effects of Therapy

Beside of longevity data, we collect day-to-day qualitative data about each animal in the study.

Regular Sampling

Blood collection, urine sampling, stool collection. See also Biochemistry and Haematology unit.

Statistical Analysis

Study results might be finalised by our bioinformaticians.

Do you have questions? Ask us

Standard services Microsurgery Service

Application of Telemetric Device

Castration

Castration of rats, mice and hamsters of both gender.

Tumour re-transplantation

Do you have questions? Ask us

Standard Services In House Multimodal Analysis

Analysis of samples please see these units: Histopathology, Biochemistry and Haematology, Immunology and Maldi.

For statistical analysis, see Bioinformatics unit.

Do you have questions? Ask us


Custom services Project Design Consulting

Maximizing the outcome on statistical minimum animals involved in a study due to multimodal approach to save your time and costs to get preliminary data, preparation and leading of robust studies and project licence support.

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Custom services Onco-immunology Studies and Xenograft Studies

Humanised models as translational platforms for immuno-oncology research focused on cancer, allergies and inflammation or Graft versus Host Disease (GvHD).
There are two models CD34+ humanized model for long-term studies for drug efficacy studies and PBMC humanized model mainly used for short-term graft rejection studies.

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