Metabolism

Metabolism

Metabolic phenotyping is part of primary rodent phenotyping pipeline. Primary screens include intraperitoneal glucose tolerance test, insulin blood level and energy expenditure measurement. These screens were developed by Eumorphia and EMPReSS, a European Mouse Phenotyping Resource for Standardized Screens. More info

Intraperitoneal glucose tolerance test and insulin blood level are two tests focused on screening for diabetic phenotype. Energy expenditure measurement using indirect calorimetry should detect more wide spectra of metabolic abnormalities besides diabetes. All three test are currently running in CCP and additional metabolic challenges based on chow diet are available upon request.

Standard services Intraperitoneal Glucose Tolerance Test (IPGTT)

This test is used for detection of abnormalities in glucose metabolism. In human medical practice this test is performed to diagnose diabetes and metabolic syndrome. The test is similar for mice and humans; it measures the clearance of a glucose load from the body at different time points. Mice are fasted for 16h, basal glucose level is measured prior to glucose intraperitoneal injection and glucose levels are measured during the following hours. This test involves live animal experimentation and therefore a “protokol pokusu” (permit for animal experiment) is required.

Insulin Blood Level

Insulin is a peptide hormone which is an important regulator of glucose homeostasis. The level of insulin in mouse plasma is detected during terminal bleeding. Together with the intraperitoneal glucose tolerance test, an abnormal level of insulin is indicator of diabetic or metabolism related phenotype.

Energy Expenditure

Indirect calorimetry is a noninvasive method where oxygen consumption and CO2 production over a given period of time, and in a home cage environment, is measured. The purpose of these measurements is to provide information about energy metabolism and to detect abnormalities of carbohydrate and lipid metabolism in rodents. Since oxygen and CO2 are measured simultaneously, the respiratory exchange ratio (RER) can be calculated. As activity and food and water intake are also monitored, total energy expenditure can be evaluated.

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Custom services Pair-feeding Studies

These studies involve an experimental setup where reduced food intake or the very same dosage of drug are explored. In drug pair feeding experiments, the amount of drug eaten by the treated group each day is determined and given to a vehicle-treated ‘pair-fed’ group of animals housed under identical conditions the following day. During the whole period food and water intake are in-line monitored together with energy expenditure monitoring. Customized setup includes choice of rodent mouse or rats, diet (control, high fat etc.), drug delivery and monitoring parameters.

High Fat Fed Rodent Model

These experiments are designed upon request of each client. The experimental design usually includes body weight, food and water intake, blood analysis for key metabolites, body composition and finally histological analysis.

Diabetes Induced in Rodent Model

Low or high doses of streptozotocin (STZ), a toxin that specifically destroys the insulin–producing β-cells of the pancreas, is used. Together with high fat diet this model mimics disturbances in patient with type 2 diabetes.

Body Composition

See our bioimaging section.

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