Immunology

Immunology

The Immunology unit is taking part in the revealing of the etiopathogenic mechanisms of the immunological diseases using the transgenic mouse model. Generally, immunological disorders comprise the immune deficiencies, allergies, inflammatory diseases and cancerogenesis as well. More info

The immune system functions are reflected in changes of the immune cell subpopulations and their products as cytokines, chemokines and other similar substances. The pathological changes can be registered in the afflicted organs, in responsible immune organs as well as in blood. We are able to evaluate the immune system functions by use of various immunological methods.

Standard services Comprehensive Immunophenotyping Panel (FACS)

We routinely perform standard phenotypic immunological characterisation of particular immune cell populations in terms of their cellularity and phenotype using multicolour flow cytometry. Currently we offer two standardized panels for lymphoid and myeloid cell lineages. Standard list of CD antigens evaluated:

PANEL A (T cells)

  • antibody dye
  • CD5 BV421
  • CD4 FITC
  • CD44 PE
  • CD8a PE-CF594
  • CD25 PE-Cy7
  • CD62L APC-C7

PANEL B (B and myeloid cells)

  • CD5 BV421
  • Ly6G BV421
  • CD19 BV510
  • Ly6C FITC
  • CD21/CD35 PE
  • CD11b PE-CF594
  • CD11c PE-Cy7
  • MHCII APC-Cy7
  • Fc block CD16/CD32 Purified
  • Live/Dead SytoxBlue

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Custom services Experimental Anti-Tumour Immunotherapy

We are open for collaborations in the field of tumour immunology and experimental anti-tumour therapy using murine models in general. We can test the efficacy of anti-tumour immuno- and chemotherapy and their combinations in various settings, using syngeneic murine models, K.O. mice, as well as tumour xenografts in immunodeficient mice. We can monitor immune responses during cancer development and therapy by a complex analysis, that involves analysis of immune cells both in the immune organs (spleen, lymph nodes) and the tumour microenvironment, cytokine levels monitoring and functional studies (cytotoxic tests, proliferation tests, immune suppression analyses) ex vivo. Notably, we have established experimental models of the minimal residual tumour disease after surgery or chemotherapy.

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