Phenotyping

Phenotyping

The Phenotyping Module at the Czech Center for Phenogenomics represents a highly standardized yet flexible platform for comprehensive in vivo functional characterization of mouse and rat models. We deliver integrated phenotyping pipelines ranging from primary systemic screening to advanced, physiology-driven and challenge-based studies, enabling precise interpretation of gene function and disease mechanisms. Our infrastructure forms a robust translational backbone for preclinical research, including customized study design and GLP-compatible workflows. More info

The Phenotyping Module provides a fully integrated platform for deep physiological and morphological characterization of experimental rodent models under controlled SPF conditions. The core strength of the module lies in its ability to combine rigorously standardized primary phenotyping pipelines with highly flexible, hypothesis-driven secondary and tertiary analyses. Such approach enables comprehensive functional interpretation of both knockout models and precise genetic variants, with a strong emphasis on rare disease modeling and translational relevance.

The module integrates advanced multimodal technologies, including:

  • Imaging: in vivo microCT, high-resolution ex vivo microCT, optical imaging, ultrasound combined with photoacoustic imaging and confocal endoscopy.
  • Physiological phenotyping: neurobehavioral assays, metabolic phenotyping, cardiovascular and respiratory measurements, and electrophysiology.
  • Oncology and translational models: patient-derived xenografts (PDX) and experimental cancer models for in vivo therapeutic testing.
  • Developmental assessment: embryonic and postnatal phenotyping, including high-resolution morphological and functional analysis.
  • Infectious disease models: studies conducted under BSL-3 conditions enabling controlled investigation of pathogenic agents.
  • Analytical platforms: clinical biochemistry, immunoassays, LC/MS-based metabolomics and lipidomics, and MALDI imaging for spatial molecular profiling.
  • Immunology and cellular profiling: flow cytometry and advanced immune phenotyping approaches.
  • Histopathology: comprehensive tissue processing, high-resolution imaging, and expert pathological evaluation.

A key advantage of multimodality is its ability to generate high-content, multidimensional datasets from individual animals, maximizing data yield while minimizing animal usage.

Importantly, the module operates as a fully customizable service environment, where experimental design is adapted to the specific scientific or translational question. This includes seamless integration into preclinical development pipelines, with the capability to deliver GLP-compatible studies, particularly in areas such as safety and toxicity assessment.

Together, the Phenotyping Module constitutes a unique, high-resolution translational core facility, bridging fundamental genetics with actionable preclinical outcomes.

To order our services go to “Directory of CCP Request Forms”