2nd Programmable Nucleases Course in April – registration open

20. 12. 2016 Education Event Information

2nd Programmable nucleases (CRISPR/Cas9) Transgenesis Course at CCP

This course, jointly provided by the Czech Centre for Phenogenomics and PHENOMIN-ICS from Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.

Course highlights:

• Design targeting strategy for your project of interest

• Apply the targeting tools and validate CRISPR activity

• Deliver the editing tools to cell lines and zygotes

• Identify CRISPR induced genomic modifications

Date: April 3-7, 2017
Place: Czech Centre for Phenogenomis, Vestec, Czech Republic
Application deadline: 15th February 2017 (results posted 20th February 2017)
Participants: A maximum of 15 participants including 2 scholarships.
Course fee: 980 € for participants from academic institutions and universities. The cost for participants from industry is available on request.

Participation on the course is subject to a selection process. Applications must be accompanied by a short CV, letter of motivation (max 200 words), and a short letter of support. Accommodation and transport costs are not included in the fee and has to be covered by participants on their own. Lunches and refreshments during the course as well as joint dinners are included.

Participants wishing to apply for the scholarship should state this clearly in the cover letter. Please note that the scholarship will cover the course fee only.

Applications should be sent to the following email address: ccp-tam@img.cas.cz

The lecturers and instructor team 

The participants of the 2nd programmable nucleases (CRISPR/Cas9) Transgenesis Course will be trained by the experienced team of people who are also equipped with the experience of organizing the “hands-on nuclease course’ accompanying the Transgenic technology meeting TT2016.

Guest lecturers:
Lluis Montoliu (CNB, Spain)
Bernd Zetsche (Broad Institute, USA)
Ronald Naumann (MPI-CBG, Dresden)

Czech Centre for Phenogenomics (IMG/BIOCEV)
Radislav Sedlacek
Björn Schuster
Inken M Beck
Jana Kopkanova
and all other members of Transgenic and Archiving Module of CCP

PHENOMIN-ICS, Strasbourg
Yann Herault
Marie-Christine Birling
Guillaume Pavlovic

 

Preliminary program (Updated PDF file)

 Day 1 – Monday 3rd April 2017

10:45 – 11:00             Arrival and Registration

11:00 – 11:15              Welcome and Introduction to the course and teaching material

Introduction of team members

                                      Radislav Sedlacek (IMG/CCP)

 11:15 – 13:15              Participants: presentation individual projects

                                       Radislav Sedlacek (IMG/CCP) and all instructors

 13:15 – 14:15              Lunch

14:15 – 14:45              Lecture: Pros-and-cons of programmable nucleases

                                      Radislav Sedlacek (IMG/CCP)

 14:45 – 16:15             Practical training session: gRNA validation I

                                      Jana Kopkanová & Björn Schuster (IMG/CCP)

 16:15 –17:00             Lecture: Historical perspective – how we came to nucleases

                                      Lluis Montoliu (CNB, Spain)

 17:00 –18:00            get together with time for discussions with the speaker and instructors

19:00                           Course Dinner 1


Day 2 – Tuesday 4th April 2017

08:00 – 08:45             Lecture: Breaking-Cas, a new guide designing program and CRISPR/Cas function

                                        Lluis Montoliu (CNB, Spain)

08:00 – 08:15              Daily agenda and outcomes overview

09:00 – 10:00               Practical training session: gRNA preparation I

                                         Jana Kopkanová & Björn Schuster (IMG/CCP)

10:00 – 10:15              Tea and Coffee

10:15 – 13:15              Practical training sessions:

  1. Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
  2. Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
  3. Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)

13:15 – 14:15              Lunch

14:15 –14:45               Lecture: gRNA validation and genotyping of Indel mutations

Björn Schuster (IMG/CCP)

14:45 – 16:15              Practical training session: gRNA preparation II

                                      Jana Kopkanová & Björn Schuster (IMG/CCP)

16:15 – 17:00             Lecture: CRISPR technology in cell culture

                                      Speaker: tba (Sigma)

 17:00 –18:00             get together with time for discussions with the speaker and instructors

18:00                           End of Day


Day 3 – Wednesday 5th April 2017

08:00 – 08:45             Lecture: repertoire of gene targeting methods in rodents

                                       Guillaume Pavlovic (PHENOMIN-ICS), Marie-Christine Birling (PHENOMIN-ICS)

08:45 – 09:00              Agenda for the day with tea and coffee

09:00 – 10:00               Practical training session: gRNA preparation III

                                         Jana Kopkanová & Björn Schuster (IMG/CCP)

10:00 – 10:15              Tea and Coffee

10:15 – 13:15              Practical training sessions:

  1. Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
  2. Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
  3. Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)

 13:15 – 14:15              Lunch

 14:15 –14:45               Lecture: Targeting vector design

Björn Schuster (IMG/CCP)

14:45 – 16:15               Practical training session: gRNA validation II

                                      Jana Kopkanová & Björn Schuster (IMG/CCP)

16:15 – 17:00              Lecture: Expanding the Research Applications for CRISPR

                                       Guillaume Pavlovic (PHENOMIN-ICS)

17:00 –18:00               get together with time for discussions with the speaker and instructors

18:00                           End of Day

 

Day 4 – Thursday 6th April 2017

08:00 – 08:45             Lecture: Lecture: Development CRISPR-Cas9 & Cpf1 technology

                                        Bernd Zetsche (Broad Institute, USA)

08:45 – 09:00              Agenda for the day with tea and coffee

09:00 – 10:00               Practical training session: gRNA validation III

                                        Jana Kopkanová & Björn Schuster (IMG/CCP)

10:00 – 10:15              TEA AND COFFEE

10:15 – 13:15              Practical training sessions:

  1. Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
  2. Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
  3. Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)

13:15 – 14:15              LUNCH

14:15 –14:45               Lecture: CRISPR/nuclease troubleshooting and genotyping

Bjorn Schuster and Jana Kopkanova (IMG/CCP)

14:45 – 16:15               Practical training session: collection of results and time for discussion/ trouble shooting

                                       All instructors

16:15 – 17:00              Lecture: genome editing using nucleases: new possibilities and application to reveal gene function in diseases

                                      Marie-Christine Birling (PHENOMIN-ICS)

17:00 –18:00              get together with time for discussions with the speaker and instructors

19:00                           Course Dinner 2

 

Day 5 – Friday 7th April 2017

08:00 – 08:45            Lecture: “from genotype to phenotype”

Yann Herault (IBGMC, Strasbourg) or invited speaker

08:45 – 09:00            Agenda for the day with tea and coffee

09:00 – 10:00            Collection of results and preparation of short presentations

                                     All instructors

 10:00 – 10:15           Tea and Coffee

 10:15 – 12:30             Short group presentations

 

Summary & Conclusions

12:30 – 13:15              LUNCH

13:15                          End of course

Optional:

13:15 – 15:00    Tour through Czech Centre for Phenogenomics (phenotyping and breeding)