canSERV open call for free state of the art services
canSERV invites applications covering the entire range of the oncology developmental pipeline; supporting discoveries in fundamental research to translational science and personalised oncology. canSERV welcomes excellent research applications addressing at least one of the four strategic goals of the Cancer Mission.
- Understanding of Cancer
- Prevention and Early Detection
- Diagnosis and treatment
- Quality of life for patients and their families
CCP provides following services under this call:
Generation of precision cancer mouse models using CRISPR-Cas9
The precision cancer mouse model generation service uses CRISPR-Cas9 technology to design precise and unique cancer mouse models. This service includes prediction of off-target sites, preparation of sgRNA’s and Cas9 mRNA/protein, and injection into zygotes to generate F0 founder mutant animals (C57BL/6N or C57BL/6J genetic background preferred). Selected F0 animals will be bred to germline to produce F1 genome edited animals. Possible allele types that can be generated are indels, exon deletions (< 10kb) and point mutation insertions. Newly developed mouse models will be made available to selected applicants within an average of 12 months following provision of all required information to start the mouse production.
In-depth cancer-based phenotyping of cancer mouse models
This specialised mouse phenotyping service is directed towards cancer mouse models and combines mouse breeding and experimental animal pathology. The mouse breeding includes the breeding, genotyping and selection of the mice that will be phenotyped. The mouse handling involves the bi-weekly palpation of the mice, measuring the tumors and checking for metastasis and overall welfare. It also involves sacrificing the mice for dissection and freezing down and storing the viable tumor pieces. A health check will be performed and tumors genotyped. The experimental animal pathology encompasses the complete necropsy of 15 mice, followed by complete histology (tissue trimming, processing, paraffin embedding, sectioning, staining with hematoxylin and eosin), and histopathology report of all abnormalities (phenotypes) of several body parts and organs
Submission deadline is 4 January 2024, 2pm CET. For more information and the call text, please visit CanSERV project website: www.canSERV.eu/Calls