Advanced techniques in immunohistochemistry and transgenesis – application closed!
Czech Centre for Phenogenomics together with Institute of Molecular Genetics offered a practical laboratory course focused on histological analysis of mouse tissue, including analysis of transgenic mouse strains. The three-day course took place at IMG from December 10 to December 12, 2014 and covered following techniques:
(1) Immunohistochemistry using paraffin sections
It includes: (i) tissues fixation (using three different techniques), processing and embedding in paraffin; (ii) antigen retrieval (comparison of two different techniques); (iii) staining with an antibody of interest
(2) Immunohistochemistry using frozen section
It includes: (i) tissues fixation and sucrose saturation, preparation of blocks for sectioning; (ii) cryosectioning; (iii) staining with an antibody of interest, comparison of results with staining of paraffin sections
(3) Lineage tracing experiments in transgenic mice
It includes: (i) labeling of living cells in mouse tissues; (ii) visualization and isolation of labeled cells
(4) Transgenic techniques in mouse and fish
Two lectures covering the newest approaches applicable for generation of transgenic mouse and fish.
The course was be held at the Histology core facility at the Institute of Molecular Genetics ASCR, Prague (http://www.img.cas.cz/core-facilities/histology-lab/) in both Czech and English. The cost of accommodation, diets and travel of all participants was be covered by project of EC OP CZ.1.07/2.3.00/30.0027 “Founding the Centre of Transgenic Technologies”.
All course materials are available for download at this link (for course participants only).