The Czech Centre for Phenogenomics is hosted by the Institute of Molecular Genetics of the Czech Academy of Sciences.
The CCP as a large research infrastructure establishes a broad range of technologies that are divided in three basic areas: animal model generation, phenotyping and experimental approaches and housing and breeding. These technology areas are covered by three CCP modules managed under the management of CCP executive board.
The daily operations of CCP are directed by the executive board consisting of the executive director of the centre, the heads or deputy heads of respective modules and the scientific director. The director and the board use dedicated project management office with a group of administrators to handle all administrative and managerial tasks. Advisory committees provides invaluable suggestions to the board.
Transgenic and Archiving Module
TAM has established a comprehensive technology portfolio for rodent model generation that is fully comparable with any world-class facility in this specific area. CCP has established routine use of technologies of TALEN and CRISPR/Cas9 assisted gene targeting and genome editing to substantially improve our services in custom-tailored targeting projects saving cost and time. Other technologies provided by TAM include embryonic stem (ES) cell derivation and manipulation, development of targeting strategies and tools, generation of transgenic animals including conditional knock-out and knock-in models, genotyping, cryo-archiving, export/import/distribution, consultancy support, and administrative support (GMO licenses).
The technologies used in the Phenotyping module constitute a whole compendium for examining major physiological systems of the animal body (biochemisty, metabolism, behavior, hearing etc) and enable collection of almost 600 parameters for each phenotyped animal. The portfolio of services and parameters is continually developing. Besides the basic phenotyping tests, CCP provides also secondary tests (challenges), which could be tailored according to the users’ needs. In addition to the service to academic users, CCP provides a contract research as a service. CCP has also established the GLP (good laboratory praxis) mode to serve companies that wish to perform pre-clinical studies in our centre (currently for histopathology and biochemistry unit).
Animal Facility Module
CCP has one of the most progressive animal facilities in Europe regarding logistics, versatility, and demand for animal health. AFM service provides mainly breeding and housing under SPF conditions, health monitoring, biopsies, administration service for import/export of rodent lines, GMO (gene-modified organism), and further administration/transportation. Technologies employed include housing/breeding animals in individually ventilated cages (IVC) and digitally ventilated cages (DVG), giving CCP a very effective way of breeding, and monitoring of animals. CCP operates in a mode of barrier system using a one-way flow of material and animals, a health monitoring system, and advanced software-assisted management of animal facility operations.
International Scientific Advisory Board of CCP
The Scientific Advisory Board (SAB) of the Czech Centre for Phenognomics (CCP) is the advisory body for the management of the infrastructure. The members of CCP SAB are worldwide recognized scientists & industry representatives determined to bring to CCP contemporary expertise and up-to-date knowledge in their respective fields. In interaction with the director of the centre Dr Radislav Sedlacek and with support of scientific director of the centre Prof. Jiri Forejt the SAB provides periodic feedback on CCP activities and advises and recommends towards the operation and future developments to ensure high quality of services, expertise as well as research projects and evaluates the efficiency of CCP operations. Besides the regular onsite meetings the SAB members provides ad hoc consultations on establishment of new research directions, introduction of new technologies and services and new major international co-operations or for future funding applications.
The SAB members are:
Prof. Rudi Balling, Director of the Luxembourg Centre for Systems Biomedicine
Prof. Martin Hrabe de Angelis, Director of the Institute for Experimental Genetics at Helmholtz Zentrum, München, director of the European Mouse Mutant Archive (EMMA), and director of the German Mouse Clinic (GMC)
Prof. Danny Huylebroeck, Stem Cell Biology and Embryology Unit, Faculty of Medicine, KU Leuven, and Professor, Stem Cell Institute (ESI) at Erasmus MC
Dr. Ralf Kühn, Group Leader iPS Cell Based Disease Modeling & Head of Transgenic Core Facility, Max Delbrück Centrum Berlin
Dr. Iva S. Morse, Vice President, Process Solutions, MilliporeSigma
(names listed in alphabetical order)
We thank to all SAB member for their invaluable support and commitment.